1 September 2006 Computer-based tracking of single sperm
Author Affiliations +
Abstract
This paper describes a robust single sperm tracking algorithm (SSTA) that can be used in laser optical trapping and sperm motility studies. The algorithm creates a region of interest (ROI) centered about a sperm selected by the user. SSTA contrast enhances the ROI image and implements a modified four-class thresholding method to extract the tracked sperm as it transitions in and out of focus. The nearest neighbor method is complemented with a speed-check feature to aid tracking in the presence of additional sperm or other particles. SSTA has a collision-detection feature for real or perceived collision or near-miss cases between two sperm. Subsequent postcollision analysis employs three criteria to distinguish the tracked sperm in the image. The efficacy of SSTA is validated through examples and comparisons to commercially available computer-aided sperm tracking systems.
Linda Z. Shi, Jaclyn Nascimento, Michael W. Berns, Elliot L. Botvinick, "Computer-based tracking of single sperm," Journal of Biomedical Optics 11(5), 054009 (1 September 2006). https://doi.org/10.1117/1.2357735
JOURNAL ARTICLE
10 PAGES


SHARE
RELATED CONTENT

A Lisp-Based PC Vision Workstation
Proceedings of SPIE (June 06 1987)
Automatic violence detection in digital movies
Proceedings of SPIE (November 01 1996)
Computer-Controlled Electrophoresis Microscope
Proceedings of SPIE (August 22 1980)

Back to Top