1 September 2006 Dual-axes confocal reflectance microscope for distinguishing colonic neoplasia
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J. of Biomedical Optics, 11(5), 054019 (2006). doi:10.1117/1.2363363
Abstract
A dual-axes confocal reflectance microscope has been developed that utilizes a narrowband laser at 1310 nm to achieve high axial resolution, image contrast, field of view, and tissue penetration for distinguishing among normal, hyperplastic, and dysplastic colonic mucosa ex vivo. Light is collected off-axis using a low numerical aperture objective to obtain vertical image sections, with 4- to 5-µm resolution, at tissue depths up to 610 µm. Post-objective scanning enables a large field of view (610×640 µm), and balanced-heterodyne detection provides sensitivity to collect vertical sections at one frame per second. System optics are optimized to effectively reject out-of-focus scattered light without use of a low-coherence gate. This design is scalable to millimeter dimensions, and the results demonstrate the potential for a miniature instrument to detect precancerous tissues, and hence to perform in vivo histopathology.
Jonathan T. C. Liu, Michael J. Mandella, Shai Friedland, Roy M. Soetikno, James M. Crawford, Christopher H. Contag, Gordon S. Kino, Thomas D. Wang, "Dual-axes confocal reflectance microscope for distinguishing colonic neoplasia," Journal of Biomedical Optics 11(5), 054019 (1 September 2006). http://dx.doi.org/10.1117/1.2363363
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KEYWORDS
Confocal microscopy

Microscopes

Reflectivity

Gaussian beams

Image resolution

Light sources

Tissue optics

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