1 November 2006 Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy
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Abstract
We attempt to characterize the degree of skin thermal damage by using multiphoton microscopy to characterize dermal thermal damage. Our results show that dermal collagen and elastic fibers display different susceptibility to thermal injury. Morphologically, dermal collagen starts to denature at 60°C while fracture and aggregation of elastic fibers do not occur until 65°C. With increasing temperatures, the structures of both elastic and collagen fibers deteriorate. While second-harmonic-generation (SHG) imaging is helpful in identifying the denaturation temperature of collagen, autofluorescence (AF) imaging can help to identify the structural alternations of tissue at higher temperatures when SHG signals have decayed. We also employ a ratiometric approach based on the AF-to-SHG index of dermis (ASID) to characterize the degree of dermal thermal damage. Use of the ASID index can bypass the difficulty in analyzing inhomogeneous dermal fibers and show that dermal collagen starts to denature at 60°C. Our results suggest that with additional developments, multiphoton microscopy has potential to be developed into an effective in vivo imaging technique to monitor and characterize dermal thermal damage.
© (2006) Society of Photo-Optical Instrumentation Engineers (SPIE)
Ming-Gu Lin, Ming-Gu Lin, Tsung-Lin Yang, Tsung-Lin Yang, Cheng-Tien Chiang, Cheng-Tien Chiang, Hsien-Ching Kao, Hsien-Ching Kao, Jin-Ning Lee, Jin-Ning Lee, Wen Lo, Wen Lo, Shiou-Hwa Jee, Shiou-Hwa Jee, Yang-Fang Chen, Yang-Fang Chen, Chen-Yuan Dong, Chen-Yuan Dong, Sung-Jan Lin, Sung-Jan Lin, } "Evaluation of dermal thermal damage by multiphoton autofluorescence and second-harmonic-generation microscopy," Journal of Biomedical Optics 11(6), 064006 (1 November 2006). https://doi.org/10.1117/1.2405347 . Submission:
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