1 January 2007 Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers
Author Affiliations +
J. of Biomedical Optics, 12(1), 014005 (2007). doi:10.1117/1.2435710
Abstract
Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence polarization imaging of the nonmelanoma cancers stained using antibiotics from the tetracycline family. To determine the feasibility of the method, fluorescence polarization images of 86 thick, fresh cancer excisions were studied. We found that the level of endogenous fluorescence polarization was much lower than that of exogenous, and that the average values of fluorescence polarization of tetracycline derivatives were significantly higher in cancerous as compared to normal tissue. Out of 86 tumors [54 stained in demeclocycline (DMN) and 32 in tetracycline (TCN)], in 79 cases (51—DMN, 28—TCN) the location, size, and shape of the lesions were identified accurately. The results of this trial indicate that nonmelanoma skin tumors can be distinguished from healthy tissue based on the differences in exogenous fluorescence polarization of TCN and/or DMN. Therefore, the developed technique can provide an important new tool for image-guided cancer surgery.
Anna N. Yaroslavsky, Elena Vladimirovna Salomatina, Victor Neel, Richard Rox Anderson, Thomas J. Flotte, "Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers," Journal of Biomedical Optics 12(1), 014005 (1 January 2007). http://dx.doi.org/10.1117/1.2435710
JOURNAL ARTICLE
9 PAGES


SHARE
KEYWORDS
Luminescence

Polarization

Tumors

Tissues

Skin

Cancer

Skin cancer

RELATED CONTENT

Ex vivo and in vitro synchrotron based micro imaging of...
Proceedings of SPIE (September 20 2010)
Polarized nature of synchrotron radiation
Proceedings of SPIE (November 01 1991)
Nanomedicine: a new paradigm in diagnosis and therapy
Proceedings of SPIE (November 17 2005)

Back to Top