1 January 2007 Visualization of hepatobiliary excretory function by intravital multiphoton microscopy
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Abstract
Intravital imaging of hepatobiliary excretion is vital for elucidating liver metabolism. In this work, we describe a novel method to observe the intravital dynamics of the uptake, processing, and excretion of an organic anion, 6-carboxyfluorescein diacetate (6-CFDA) in the hepatobiliary system. This is achieved by the use of multiphoton microscopy and an intravital hepatic imaging chamber. The high-quality images show sequential uptake and processing of 6-CFDA from the hepatocytes and the subsequent excretion into bile canaliculi within approximately 50 min. This is a promising technique to study intravital hepatic physiology and metabolism.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yuan Liu, Yuan Liu, Hsiao-Ching Chen, Hsiao-Ching Chen, Shu-Mei Yang, Shu-Mei Yang, Tzu-Lin Sun, Tzu-Lin Sun, Wen Lo, Wen Lo, Ling-Ling Chiou, Ling-Ling Chiou, Guan-Tarn Huang, Guan-Tarn Huang, Chen-Yuan Dong, Chen-Yuan Dong, Hsuan-Shu Lee, Hsuan-Shu Lee, } "Visualization of hepatobiliary excretory function by intravital multiphoton microscopy," Journal of Biomedical Optics 12(1), 014014 (1 January 2007). https://doi.org/10.1117/1.2710237 . Submission:
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