1 January 2007 High-resolution whole organ imaging using two-photon tissue cytometry
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Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer's disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Timothy Ragan, Timothy Ragan, Jeremy D. Sylvan, Jeremy D. Sylvan, Ki H. Kim, Ki H. Kim, Hayden Huang, Hayden Huang, Karsten Bahlmann, Karsten Bahlmann, Richard T. Lee, Richard T. Lee, Peter T. C. So, Peter T. C. So, } "High-resolution whole organ imaging using two-photon tissue cytometry," Journal of Biomedical Optics 12(1), 014015 (1 January 2007). https://doi.org/10.1117/1.2435626 . Submission:

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