1 January 2007 Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection
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J. of Biomedical Optics, 12(1), 014038 (2007). doi:10.1117/1.2437143
Abstract
A highly parallel microarray scanner for functional genomic research using a dynamically reconfigurable holographic excitation and hyperspectral fluorescence detection is described. The light from two laser sources (405 and 532 nm) is split into an arbitrary number of focused spots on the biochip using a pair of spatial light modulators and a novel imaging system. The parallel optical scanner includes a hyperspectral detection unit with a high-sensitivity CCD camera to detect and analyze the emitted fluorescence spectra (from 430 to 800 nm) of all illuminated spots simultaneously. By using an xy scanner, the spectra of all spots on an entire array can be read out line by line.
Carsten Glasenapp, Wolfgang Monch, Holger Krause, Hans Zappe, "Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection," Journal of Biomedical Optics 12(1), 014038 (1 January 2007). http://dx.doi.org/10.1117/1.2437143
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KEYWORDS
Luminescence

Holography

Fluorescence spectroscopy

CCD cameras

LCDs

Signal detection

CCD image sensors

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