1 March 2007 Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis
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Abstract
The purpose of this study is to demonstrate the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for the ex-vivo visualization of human corneal morphological alterations due to infectious processes. The structural alterations of both cellular and collagenous components can be respectively demonstrated using fluorescence and SHG imaging. In addition, pathogens with fluorescence may be identified within turbid specimens. Our results show that multiphoton microscopy is effective for identifying structural alterations due to corneal infections without the need of histological processing. With additional developments, multiphoton microscopy has the potential to be developed into an imaging technique effective in the clinical diagnosis and monitoring of corneal infections.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Hsian-Yuan Tan, Yen Sun, Wen Lo, Shu-Wen Teng, Ruei-Jr Wu, Shiou-Hwa Jee, Wei-Chou Lin, Ching-Hsi Hsiao, Hsin-Chiung Lin, Yeong-Fong Chen, David Hui-Kang Ma, Samuel Chao-Ming Huang, Sung-Jan Lin, Chen-Yuan Dong, "Multiphoton fluorescence and second harmonic generation microscopy for imaging infectious keratitis," Journal of Biomedical Optics 12(2), 024013 (1 March 2007). https://doi.org/10.1117/1.2717133 . Submission:
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