1 March 2007 Analysis of the dispersion compensation of acousto-optic deflectors used for multiphoton imaging
Author Affiliations +
J. of Biomedical Optics, 12(2), 024015 (2007). doi:10.1117/1.2714061
Abstract
The acousto-optic deflector (AOD) is highly preferred in laser scanning microscopy for its fast scanning ability and random-addressing capability. However, its application in two-photon microscopy is frustrated by the dispersion of the AOD, which results in beam distortion and pulse lengthening. We report the analysis of simultaneous compensation for the angular dispersion and temporal dispersion of the AOD by merely introducing a single dispersive element such as a prism or a grating. Besides serving as a scanner, the AOD is also a part of the compressor pair by integrating the dispersive nature of the AO interaction. This compensation principle is effective for both one-dimensional (1-D) AOD and two-dimensional (2-D) AOD scanning. Switching from a 1-D to a 2-D system requires proper optical alignment with the compensation element, but does not involve any new components. Analytical expressions are given to illustrate the working principle and to help with understanding the design of the system. Fluorescence images of beads and cells are shown to demonstrate the performance of two-photon microscopy when applying this compensated 2-D AOD as scanner.
Shaoqun Zeng, Xiaohua Lv, Kun Bi, Chen Zhan, Derong Li, Wei R. Chen, Wenhui Xiong, Steven L. Jacques, Qingming Luo, "Analysis of the dispersion compensation of acousto-optic deflectors used for multiphoton imaging," Journal of Biomedical Optics 12(2), 024015 (1 March 2007). http://dx.doi.org/10.1117/1.2714061
JOURNAL ARTICLE
7 PAGES


SHARE
KEYWORDS
Prisms

Bragg cells

Acousto-optics

Adaptive optics

Multiphoton microscopy

Scanners

Biological research

RELATED CONTENT

Efficient First Stokes Generation Using A Raman Oscillator
Proceedings of SPIE (October 26 1989)
Refractive beam steering
Proceedings of SPIE (September 30 2004)
A Method To Explore Three Dimensional Absorption In Polymers
Proceedings of SPIE (December 20 1985)
Performance of PYRAMIR detector system
Proceedings of SPIE (October 25 2004)
Color diagram based on the human cone fundamentals
Proceedings of SPIE (August 14 2001)

Back to Top