1 March 2007 Fiber optic biosensor for the detection of C-reactive protein and the study of protein binding kinetics
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J. of Biomedical Optics, 12(2), 024025 (2007). doi:10.1117/1.2714029
Abstract
Application of a fiber optic biosensor (FOB) to the real-time investigation of the interaction kinetics between FITC-conjugated monoclonal sheep anti-human C-reactive protein (CRP) antibody and CRP isoforms on the surface of optical fiber is described. Recently, both the native pentameric CRP (pCRP), an acute phase protein belonging to pentraxin family, and an isoform of pCRP, modified CRP (mCRP), have been suggested to have proinflammation effects on vascular cells in acute myocardial infarction (AMI). In current studies, we generate mCRP from pCRP, and use several methods including fluorescence spectral properties, circular dichroism, analytical ultracentrifuge, and Western blotting to demonstrate their differences in physical and chemical properties as well as the purity of pCRP and mCRP. In addition, we design and implement an FOB to study the real-time qualitative and quantitative biomolecular recognition of CRP isoforms. Specifically, the association and dissociation rate constants of the reaction between FITC-conjugated monoclonal sheep anti-human CRP antibody and the pCRP and mCRP are determined. The feasibility of our current approach to measure the association and dissociation rate constants of the reaction between tested CRP isoforms was successfully demonstrated.
Chien Chou, Hsien-Yeh Hsu, Hsieh-Ting Wu, Kai-Yu Tseng, Arthur E. T. Chiou, Chih-Jen Yu, Zheng-Yuan Lee, Tsu-Shin Chan, "Fiber optic biosensor for the detection of C-reactive protein and the study of protein binding kinetics," Journal of Biomedical Optics 12(2), 024025 (1 March 2007). https://doi.org/10.1117/1.2714029
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