1 March 2007 Mirror slides for high-sensitivity cell and tissue fluorescence imaging
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J. of Biomedical Optics, 12(2), 024030 (2007). doi:10.1117/1.2722730
Abstract
Fluorescence microscopy has become the method of choice in the majority of life-science applications. We describe development and use of mirror slides to significantly enhance the fluorescence signal using standard air microscope objectives. This technique offers sufficient gain to achieve high-sensitivity imaging, together with wide field of observation and large depth of focus, two major breakthroughs for routine analysis and high-throughput screening applications on cells and tissue samples.
Eric Le Moal, Emmanuel Fort, Sandrine Lévêque-Fort, Anne Janin, Hideyuki Murata, Fabrice P. Cordelieres, Marie-Pierre P. Fontaine-Aupart, Christian Ricolleau, "Mirror slides for high-sensitivity cell and tissue fluorescence imaging," Journal of Biomedical Optics 12(2), 024030 (1 March 2007). http://dx.doi.org/10.1117/1.2722730
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KEYWORDS
Mirrors

Luminescence

Objectives

Tissues

Microscopes

Image filtering

Microscopy

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