1 May 2007 Non-contact detection of ciprofloxacin in a model anterior chamber using Raman spectroscopy
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J. of Biomedical Optics, 12(3), 034005 (2007). doi:10.1117/1.2737385
Abstract
We examine the application of an improved noncontact and noninvasive Raman spectroscopic technique in measuring medicines at therapeutic concentrations in a model system mimicking the anterior chamber of the eye. A 90-deg laser Raman scattering geometry is employed to reduce the direct exposure of the basic cordial ocular tissues to the laser beam and increase the signal-to-noise ratio of the spectra. The technique is applied to a commercially available artificial anterior chamber (AAC) fitted with corneas of porcine eyes. Specific Raman signatures of ciprofloxacin (Ciproxin®), a fluoroquinolone based antibiotic, have been resolved. Last, a partial least-squares (PLS) chemometric algorithm has been developed to predict the concentration of ciprofloxacin in AAC over the range from 0 to 1 mg/mL with a correlation coefficient R2=98.4% and an RMS error of prediction equal to 41 μg/mL.
Th. Sideroudi, Nikolaos Pharmakakis, A. Tyrovolas, G. Papatheodorou, G.D. Chryssikos, G.A. Voyiatzis, "Non-contact detection of ciprofloxacin in a model anterior chamber using Raman spectroscopy," Journal of Biomedical Optics 12(3), 034005 (1 May 2007). https://doi.org/10.1117/1.2737385
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