1 May 2007 Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo
Author Affiliations +
J. of Biomedical Optics, 12(3), 034007 (2007). doi:10.1117/1.2737351
Abstract
An effective cancer control strategy requires improved early detection methods, patient-specific drug selection, and the ability to assess response to targeted therapeutics. Recently, plasmon resonance coupling between closely spaced metal nanoparticles has been used to develop ultrasensitive bioanalytical assays in vitro. We demonstrate the first in vivo application of plasmon coupling for molecular imaging of carcinogenesis. We describe molecular-specific gold bioconjugates to image epidermal growth factor receptor (EGFR); these conjugates can be delivered topically and imaged noninvasively in real time. We show that labeling with gold bioconjugates gives information on the overexpression and nanoscale spatial relationship of EGF receptors in cell membranes, both of which are altered in neoplasia. EGFR-mediated aggregation of gold nanoparticles in neoplastic cells results in more than a 100-nm color shift and a contrast ratio of more than tenfold in images of normal and precancerous epithelium in vivo, dramatically increasing contrast beyond values reported previously for antibody-targeted fluorescent dyes.
Jesse S. Aaron, Nitin Nitin, Kort Travis, Sonia Kumar, Thomas G. Collier, Sun Young Park, Miguel Jose Yacaman, Lezlee Coghlan, Michelle Follen, Rebecca R. Richards-Kortum, Konstantin V. Sokolov, "Plasmon resonance coupling of metal nanoparticles for molecular imaging of carcinogenesis in vivo," Journal of Biomedical Optics 12(3), 034007 (1 May 2007). http://dx.doi.org/10.1117/1.2737351
JOURNAL ARTICLE
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KEYWORDS
Nanoparticles

Gold

Reflectivity

Particles

Scattering

Biopsy

In vivo imaging

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