1 May 2007 Scattering suppression and confocal detection in multifocal multiphoton microscopy
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We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality.
Jörg Martini, Volker Andresen, Dario Anselmetti, "Scattering suppression and confocal detection in multifocal multiphoton microscopy," Journal of Biomedical Optics 12(3), 034010 (1 May 2007). https://doi.org/10.1117/1.2736425

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