1 May 2007 Scattering suppression and confocal detection in multifocal multiphoton microscopy
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Abstract
We have developed a new descanned parallel (32-fold) pinhole and photomultiplier detection array for multifocal multiphoton microscopy that effectively reduces the blurring effect originating from scattered fluorescence photons in strongly scattering biological media. With this method, we achieve a fourfold improvement in photon statistics for detecting ballistic photons and an increase in spatial resolution by 21% in the lateral and 35% in the axial direction compared to single-beam non-descanned multiphoton microscopy. The new detection concept has been applied to plant leaves and pollen grains to verify the improvements in imaging quality.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jörg Martini, Volker Andresen, Dario Anselmetti, "Scattering suppression and confocal detection in multifocal multiphoton microscopy," Journal of Biomedical Optics 12(3), 034010 (1 May 2007). https://doi.org/10.1117/1.2736425 . Submission:
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