1 May 2007 Two-color in vivo dynamic contrast-enhanced pharmacokinetic imaging
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Optical imaging is unique among in vivo imaging methods because it is possible to simultaneously resolve two or more probes emitting at different wavelengths of light. We employed two near-infrared (NIR) fluorescent optical probes, each labeled with a different protein, to simultaneously evaluate the pharmacokinetics of each probe. Dynamic optical imaging was performed in live mice after the coinjection of bovine serum albumin (BSA) and galactosamine-conjugated bovine serum albumin (GmSA) labeled with either Cy5.5 or Cy7 NIR dyes. The pharmacokinetics of BSA and GmSA were independently and simultaneously visualized. Next, two-color dynamic imaging of biotinylated BSA (b-BSA) and BSA labeled with Cy5.5 or Cy7 was performed before and after an avidin "chase." Following avidin injection, fluorescently labeled b-BSA rapidly accumulated in the liver, while minimal liver uptake of BSA was noted. Thus, multicolor dynamic contrast-enhanced optical imaging can be performed to noninvasively track the pharmacokinetics of different proteins. This imaging technique can be applied to a wide variety of optically labeled proteins in order to simultaneously track their biodistribution.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Yukihiro Hama, Yukihiro Hama, Yoshinori Koyama, Yoshinori Koyama, Peter L. Choyke, Peter L. Choyke, Hisataka Kobayashi, Hisataka Kobayashi, } "Two-color in vivo dynamic contrast-enhanced pharmacokinetic imaging," Journal of Biomedical Optics 12(3), 034016 (1 May 2007). https://doi.org/10.1117/1.2745306 . Submission:

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