1 May 2007 New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons
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Abstract
Cameleons are genetically encoded fluorescence resonance energy transfer (FRET)–based Ca2+ indicators. Attempts to use cameleons to detect neural activity in vertebrate systems have been largely frustrated by the small FRET signal, in contradistinction to the higher signals seen in Drosophila and Caenorhabditis elegans. We have developed a statistical optimization method capable of detecting small ratiometric signals in noisy imaging data, called statistical optimization for the analysis of ratiometric signals. Using this method, we can detect and estimate anticorrelated ratiometric signals with subcellular resolution in cultured, dissociated zebrafish spinal neurons expressing cameleon or loaded with fluo-4 and fura-red. This method may make it possible to use yellow cameleons for measuring neural activity at high resolution in transgenic animals.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Xiang Fan, Xiang Fan, Anirban Majumder, Anirban Majumder, Sean S. Reagin, Sean S. Reagin, Erika L. Porter, Erika L. Porter, Andrew T. Sornborger, Andrew T. Sornborger, Charles H. Keith, Charles H. Keith, James D. Lauderdale, James D. Lauderdale, } "New statistical methods enhance imaging of cameleon fluorescence resonance energy transfer in cultured zebrafish spinal neurons," Journal of Biomedical Optics 12(3), 034017 (1 May 2007). https://doi.org/10.1117/1.2745263 . Submission:
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