1 May 2007 Quantitative comparison of tissue oxygen and motexafin lutetium uptake by ex vivo and noninvasive in vivo techniques in patients with intraperitoneal carcinomatosis
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J. of Biomedical Optics, 12(3), 034023 (2007). doi:10.1117/1.2743082
Abstract
(PDT). This technique has been extensively validated in tissue phantoms; however, validation in patients has been limited. This pilot study compares blood oxygenation and photosensitizer tissue uptake measured by multiwavelength DRS with ex vivo assays of the hypoxia marker, 2-(2-nitroimida-zol-1[H]-yl)-N-(2,2,3,3,3-pentafluoropropyl)acetamide (EF5), and the photosensitizer (motexafin lutetium, MLu) from tissues at the same tumor site of three tumors in two patients with intra-abdominal cancers. Similar in vivo and ex vivo measurements of MLu concentration are carried out in murine radiation-induced fibrosarcoma (RIF) tumors (n=9). The selection of optimal DRS wavelength range and source-detector separations is discussed and implemented, and the association between in vivo and ex vivo measurements is examined. The results demonstrate a negative correlation between blood oxygen saturation (StO2) and EF5 binding, consistent with published relationships between EF5 binding and electrode measured pO2, and between electrode measured pO2 and StO2. A tight correspondence is observed between in vivo DRS and ex vivo measured MLu concentration in the RIF tumors; similar data are positively correlated in the human intraperitoneal tumors. These results further demonstrate the potential of in vivo DRS measurements in clinical PDT.
Hsing-Wen Wang, Jarod C. Finlay, Kijoon Lee, Timothy C. Zhu, Mary P. Putt, Eli Glatstein, Cameron J. Koch, Sydney M. Evans, Stephen M. Hahn, Theresa M. Busch, Arjun G. Yodh, "Quantitative comparison of tissue oxygen and motexafin lutetium uptake by ex vivo and noninvasive in vivo techniques in patients with intraperitoneal carcinomatosis," Journal of Biomedical Optics 12(3), 034023 (1 May 2007). http://dx.doi.org/10.1117/1.2743082
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KEYWORDS
Tissues

Tumors

In vivo imaging

Tissue optics

Oxygen

Reflectivity

Photodynamic therapy

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