1 May 2007 Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm
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Abstract
Angle-resolved signals of polarized light scattered by biological cells provide rich information on cell morphology. Quantitative study of these signals can lead to new methods to develop and improve high-throughput instrumentation for cell probing such as scattering-based flow cytometry. We employ a goniometer system with a photoelastic modulation scheme to determine selected Mueller matrix elements of B-cell hydrosol samples. The angular dependence of S11, S12, and S34 is determined from the scattered light signals between 10 and 160 deg at the three wavelengths 442, 633, and 850 nm. A finite-difference, time-domain (FDTD) method and coated-sphere model are used to investigate the effect of nuclear refractive index on the angle-resolved Mueller elements at different wavelengths using the 3-D structures of selected B cells reconstructed from confocal images. With these results, we demonstrate the value of the light-scattering method in obtaining the cell morphology information.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Huafeng Ding, Huafeng Ding, Jun Q. Lu, Jun Q. Lu, Robert Scott Brock, Robert Scott Brock, Thomas J. McConnelll, Thomas J. McConnelll, Jenifer F. Ojeda, Jenifer F. Ojeda, Kenneth Jacobs, Kenneth Jacobs, Xin-Hua Hu, Xin-Hua Hu, } "Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm," Journal of Biomedical Optics 12(3), 034032 (1 May 2007). https://doi.org/10.1117/1.2749730 . Submission:
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