In vivo dynamic light scattering imaging of blood coagulation

Vyacheslav Kalchenko; Alexander G. Brill; Michael Bayewitch; Ilya Fine; Vladimir P. Zharov; Ekaterina I. Galanzha; Valery Viktorovich Tuchin; Alon Harmelin

doi:10.1117/1.2778695

1 September 2007 In vivo dynamic light scattering imaging of blood coagulation

Vyacheslav Kalchenko; Alexander G. Brill; Michael Bayewitch; Ilya Fine; Vladimir P. Zharov; Ekaterina I. Galanzha; Valery Viktorovich Tuchin; Alon Harmelin

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J. of Biomedical Optics, 12(5), 052002 (2007). https://doi.org/10.1117/1.2778695

Abstract

Physiological blood coagulation/clotting is an essential biological process that is initiated by vessel injury and includes a cascade of enzymatic reactions finalized by fibrin polymerization and clot formation. We utilize dynamic light scattering (DLS) imaging to monitor in vivo red cell mobility as an indicator of blood coagulation. In the course of the experiments, blood flow is arrested using mechanical occlusion, and then laser injury is applied. We demonstrate that the combination of laser injury with DLS imaging on occluded blood vessels (i.e., under static conditions) is suitable to detect even subtle changes of plasma viscosity in the circulatory system, which reflects the process of clot development. This approach is noninvasive and has a relatively simple and easy-to-use technical design. Thus, the proposed methodology provides a promising tool for investigating blood clotting within the vasculature.

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Vyacheslav Kalchenko, Vyacheslav Kalchenko, Alexander G. Brill, Alexander G. Brill, Michael Bayewitch, Michael Bayewitch, Ilya Fine, Ilya Fine, Vladimir P. Zharov, Vladimir P. Zharov, Ekaterina I. Galanzha, Ekaterina I. Galanzha, Valery Viktorovich Tuchin, Valery Viktorovich Tuchin, Alon Harmelin, Alon Harmelin, } "In vivo dynamic light scattering imaging of blood coagulation," Journal of Biomedical Optics 12(5), 052002 (1 September 2007). https://doi.org/10.1117/1.2778695 . Submission:

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