1 September 2007 Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy
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Abstract
A method for the determination of the integral refractive index of living cells in suspension by digital holographic microscopy is described. Digital holographic phase contrast images of spherical cells in suspension are recorded, and the radius as well as the integral refractive index are determined by fitting the relation between cell thickness and phase distribution to the measured phase data. The algorithm only requires information about the refractive index of the suspension medium and the image scale of the microscope system. The specific digital holographic microscopy advantage of subsequent focus correction allows a simultaneous investigation of cells in different focus planes. Results obtained from human pancreas and liver tumor cells show that the integral cellular refractive index decreases with increasing cell radius.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Björn Kemper, Björn Kemper, Sebastian Kosmeier, Sebastian Kosmeier, Patrik Langehanenberg, Patrik Langehanenberg, Gert von Bally, Gert von Bally, Ilona Bredebusch, Ilona Bredebusch, Wolfram Domschke, Wolfram Domschke, Jürgen Schnekenburger, Jürgen Schnekenburger, } "Integral refractive index determination of living suspension cells by multifocus digital holographic phase contrast microscopy," Journal of Biomedical Optics 12(5), 054009 (1 September 2007). https://doi.org/10.1117/1.2798639 . Submission:
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