1 November 2007 Time dependence of singlet oxygen luminescence provides an indication of oxygen concentration during oxygen consumption
Author Affiliations +
Abstract
Singlet oxygen plays a major role in photodynamic inactivation of tumor cells or bacteria. Its efficacy depends critically on the oxygen concentration [O2], which can decrease in case oxygen is consumed caused by oxidative reactions. When detecting singlet oxygen directly by its luminescence at 1270 nm, the course of the luminescence signal is critically affected by [O2]. Thus, it should be feasible to monitor oxygen consumption during photo-oxidative processes. Singlet oxygen was generated by exciting a photosensitizer (TMPyP) in aqueous solution (H2O or D2O) of albumin. Chromatography shows that most of the TMPyP molecules are unbound, and therefore singlet oxygen molecules can diffuse in the solution. A sensor device for oxygen concentration revealed a rapid decrease of [O2] (oxygen depletion) in the solution during irradiation. The extent of oxygen depletion in aqueous albumin solution depends on the radiant exposure and the solvent. When detecting the luminescence signal of singlet oxygen, the shape of the luminescence signal significantly changed with irradiation time. Thus, local oxygen consumption could be monitored during photodynamic action by evaluating the course of singlet oxygen luminescence.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jürgen Baier, Jürgen Baier, Tim Maisch, Tim Maisch, Johannes Regensburger, Johannes Regensburger, Maria Loibl, Maria Loibl, Rudolf Vasold, Rudolf Vasold, Wolfgang Baeumler, Wolfgang Baeumler, } "Time dependence of singlet oxygen luminescence provides an indication of oxygen concentration during oxygen consumption," Journal of Biomedical Optics 12(6), 064008 (1 November 2007). https://doi.org/10.1117/1.2821153 . Submission:
JOURNAL ARTICLE
7 PAGES


SHARE
Back to Top