1 November 2007 Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo
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Abstract
Our purpose is to evaluate intramucosal gastric pH video imaging by 2′,7′-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) fluorescence ratio techniques. We use a video endoscopic imaging system and BCECF as the pH fluorescent probe. Systemic in vivo pH variations are studied in 10 pigs: five in the control group and five with respiratory acidosis induced through rebreathing. The intramucosal pH of the gastric wall is measured every 5 s and the results demonstrate a good correlation (pearson correlation=0.832) between blood gases pH measurements and pH measured with the video endocopic imaging system. Our results confirm the feasibility of using BCECF fluorescence pH imaging to measure intramucosal pH in vivo.
© (2007) Society of Photo-Optical Instrumentation Engineers (SPIE)
Philippe Rochon, Philippe Rochon, Mercé Jourdain, Mercé Jourdain, Jacques Mangalaboyi, Jacques Mangalaboyi, Francois Fourrier, Francois Fourrier, Sylvie Soulie-Begu, Sylvie Soulie-Begu, Bruno Buys, Bruno Buys, Guy Dehlin, Guy Dehlin, Jean Claude Lesage, Jean Claude Lesage, Marie Christine Chambrin, Marie Christine Chambrin, Serge R. Mordon, Serge R. Mordon, } "Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo," Journal of Biomedical Optics 12(6), 064014 (1 November 2007). https://doi.org/10.1117/1.2821698 . Submission:
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