1 November 2007 Simultaneous dynamic optical and electrical properties of endothelial cell attachment on indium tin oxide bioelectrodes
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J. of Biomedical Optics, 12(6), 064028 (2007). doi:10.1117/1.2821407
Abstract
This study quantifies the dynamic attachment and spreading of porcine pulmonary artery endothelial cells (PPAECs) on optically thin, indium tin oxide (ITO) biosensors using simultaneous differential interference contrast microscopy (DICM) and electrical microimpedance spectroscopy. A lock-in amplifier circuit monitored the impedance of PPAECs cultivated on the transparent ITO bioelectrodes as a function of frequency between 10 Hz and 100 kHz and as a function of time, while DICM images were simultaneously acquired. A digital image processing algorithm quantified the cell-covered electrode area as a function of time. The results of this study show that the fraction of the cell-covered electrode area is in qualitative agreement with the electrical impedance during the attachment phase following the cell settling on the electrode surface. The possibility of several distinctly different states of electrode coverage and cellular attachment giving rise to similar impedance signals is discussed.
Chang K. Choi, Anthony E. English, Kenneth D. Kihm, Charles H. Margraves, "Simultaneous dynamic optical and electrical properties of endothelial cell attachment on indium tin oxide bioelectrodes," Journal of Biomedical Optics 12(6), 064028 (1 November 2007). http://dx.doi.org/10.1117/1.2821407
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KEYWORDS
Electrodes

Resistance

Image processing

Image filtering

Biomedical optics

Microscopy

Digital image processing

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