1 September 2008 Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery
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J. of Biomedical Optics, 13(5), 050508 (2008). doi:10.1117/1.2997375
Abstract
The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.
Lingfeng Yu, Samarendra Kumar Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, Michael W. Berns, "Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery," Journal of Biomedical Optics 13(5), 050508 (1 September 2008). http://dx.doi.org/10.1117/1.2997375
Submission: Received ; Accepted
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KEYWORDS
Laser microsurgery

Microscopy

Laser systems engineering

Camera shutters

Phase imaging

Objectives

Mirrors

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