1 January 2008 Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier
Author Affiliations +
J. of Biomedical Optics, 13(1), 011009 (2008). doi:10.1117/1.2830656
The quantitative accuracy of fluorescence and bioluminescence imaging of small animals can be improved by knowledge of the in situ optical properties of each animal. Obtaining in situ optical property maps is challenging, however, due to short propagation distances, requirements for high dynamic range, and the need for dense spatial, temporal, and spectral sampling. Using an ultrafast gated image intensifier and a pulsed laser source, we have developed a small animal diffuse optical tomography system with multiple synthetic modulation frequencies up to >1 GHz. We show that amplitude and phase measurements with useful contrast-to-noise ratios can be obtained for modulation frequencies over the range of ~250 to 1250 MHz. Experiments with tissue simulating phantoms demonstrate the feasibility of reconstructing the absorption and scattering optical properties in a small animal imaging system.
Sachin V. Patwardhan, Joseph P. Culver, "Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier," Journal of Biomedical Optics 13(1), 011009 (1 January 2008). http://dx.doi.org/10.1117/1.2830656

Optical properties




Diffuse optical tomography

Fluorescence tomography

Tissue optics


Soft x-ray imager (SXI) onboard ASTRO-H
Proceedings of SPIE (July 29 2010)
An optical shutter for the Euclid imager
Proceedings of SPIE (July 22 2010)
Imaging through the diffusion equation
Proceedings of SPIE (March 15 1996)
Analysis of far field distance of LED arrays based on...
Proceedings of SPIE (September 18 2013)

Back to Top