1 January 2008 Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier
Author Affiliations +
J. of Biomedical Optics, 13(1), 011009 (2008). doi:10.1117/1.2830656
Abstract
The quantitative accuracy of fluorescence and bioluminescence imaging of small animals can be improved by knowledge of the in situ optical properties of each animal. Obtaining in situ optical property maps is challenging, however, due to short propagation distances, requirements for high dynamic range, and the need for dense spatial, temporal, and spectral sampling. Using an ultrafast gated image intensifier and a pulsed laser source, we have developed a small animal diffuse optical tomography system with multiple synthetic modulation frequencies up to >1 GHz. We show that amplitude and phase measurements with useful contrast-to-noise ratios can be obtained for modulation frequencies over the range of ~250 to 1250 MHz. Experiments with tissue simulating phantoms demonstrate the feasibility of reconstructing the absorption and scattering optical properties in a small animal imaging system.
Sachin V. Patwardhan, Joseph P. Culver, "Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier," Journal of Biomedical Optics 13(1), 011009 (1 January 2008). http://dx.doi.org/10.1117/1.2830656
JOURNAL ARTICLE
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KEYWORDS
Optical properties

Scattering

Absorption

Modulation

Diffuse optical tomography

Fluorescence tomography

Tissue optics

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