1 March 2008 Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin
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J. of Biomedical Optics, 13(2), 021102 (2008). doi:10.1117/1.2899149
Abstract
Accelerated diffusion of water and hyperosmotic optical clearing agents is studied as a result of enhanced epidermal permeability. A lattice of microzones (islets) of damage in stratum corneum is induced using a flash-lamp appliqué system. An optical clearing agent composed of 88% glycerol in aqueous solution is used for all experiments. Research of skin dehydration and glycerol delivery through epidermis at both intact and perforated stratum corneum is presented. The dehydration process induced by both stimuli of evaporation and osmotic agent action is studied by weight measurements. Dynamics of refractive index alteration of both glycerol solution and water during their interaction with skin samples is monitored. The amounts of water escaping from skin through the stratum corneum, due to hyperosmotic-agent action, and glycerol penetrating through the skin sample, are estimated. The results show that the proposed method allows for effective transepidermal water loss and delivery of optical clearing agents.
Elina A. Genina, Alexey N. Bashkatov, Anastasiya A. Korobko, Elena A. Zubkova, Valery Viktorovich Tuchin, Ilya V. Yaroslavsky, Gregory B. Altshuler, "Optical clearing of human skin: comparative study of permeability and dehydration of intact and photothermally perforated skin," Journal of Biomedical Optics 13(2), 021102 (1 March 2008). http://dx.doi.org/10.1117/1.2899149
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KEYWORDS
Skin

Water

Refractive index

Tissues

Optical clearing

Tissue optics

Diffusion

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