1 March 2008 Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging
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J. of Biomedical Optics, 13(2), 021105 (2008). doi:10.1117/1.2899153
Abstract
By use of a Fourier transform infrared (FTIR) spectroscopic imaging technique, we examine the dynamic optical clearing processes occurring in hyperosmotically biocompatible agents penetrating into skin tissue in vitro. The sequential collection of images in a time series provides an opportunity to assess penetration kinetics of dimethyl sulphoxide (DMSO) and glycerol beneath the surface of skin tissue over time. From 2-D IR spectroscopic images and 3-D false color diagrams, we show that glycerol takes at least 30 min to finally penetrate the layer of epidermis, while DMSO can be detected in epidermis after only 4 min of being topically applied over stratum corneum sides of porcine skin. The results demonstrate the potential of a FTIR spectroscopic imaging technique as an analytical tool for the study of dynamic optical clearing effects when the bio-tissue is impregnated by hyperosmotically biocompatible agents such as glycerol and DMSO.
Jingying Jiang, Matthias Boese, Paul H. Turner, Ruikang Wang, "Penetration kinetics of dimethyl sulphoxide and glycerol in dynamic optical clearing of porcine skin tissue in vitro studied by Fourier transform infrared spectroscopic imaging," Journal of Biomedical Optics 13(2), 021105 (1 March 2008). http://dx.doi.org/10.1117/1.2899153
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KEYWORDS
Skin

Imaging spectroscopy

Spectroscopy

FT-IR spectroscopy

Tissue optics

Infrared spectroscopy

Optical clearing

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