1 March 2008 Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue
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J. of Biomedical Optics, 13(2), 021108 (2008). doi:10.1117/1.2892687
Abstract
The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p=0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245).
Matthew V. Schulmerich, Jacqueline H. Cole, Kathryn A. Dooley, Michael D. Morris, Jaclynn Kreider, Steven A. Goldstein, "Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue," Journal of Biomedical Optics 13(2), 021108 (1 March 2008). http://dx.doi.org/10.1117/1.2892687
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KEYWORDS
Bone

Raman spectroscopy

Optical clearing

Skin

Tissue optics

Signal to noise ratio

Collagen

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