1 March 2008 Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography
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Abstract
We demonstrate the ability of multiple forms of optical coherence tomography (OCT) in the frequency domain to quantitatively size scatterers. Combined with a variety of distinct phantoms, we gain insight into the measurement uncertainties associated with using scattering spectra to size scatterers. We size spherical scatterers on a surface using swept-source OCT with an analysis based on a simple slab-mode resonance model. Automating this technique, a two-dimensional (2-D) image is created by raster scanning across a surface phantom designed to have a distinct size transition to demonstrate accuracy and repeatability. We also investigate the potential of a novel sphere-nanotube structure as a quantitative calibration artifact for use in comparing measured intensity and phase scattering spectra directly to Mie theory predictions. In another experiment, we demonstrate tissue-relevant sizing of scatterers as small as 5 μm on a surface by use of a Fourier domain OCT system with 280 nm of bandwidth from a supercontinuum source. We perform an uncertainty analysis for our high-resolution sizing system, estimating a sizing error of 9% for measurements of spheres with a diameter of 15 μm. With appropriate modifications, our uncertainty analysis has general applicability to other sizing techniques utilizing scattering spectra.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Tasshi Dennis, Tasshi Dennis, Shellee D. Dyer, Shellee D. Dyer, Andrew Dienstfrey, Andrew Dienstfrey, Gurpreet Singh, Gurpreet Singh, Paul Rice, Paul Rice, } "Analyzing quantitative light scattering spectra of phantoms measured with optical coherence tomography," Journal of Biomedical Optics 13(2), 024004 (1 March 2008). https://doi.org/10.1117/1.2899150 . Submission:
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