1 March 2008 Quantitative phase microscopy through differential interference imaging
Author Affiliations +
Abstract
An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Sharon V. King, Sharon V. King, Ariel R. Libertun, Ariel R. Libertun, Rafael Piestun, Rafael Piestun, Carol J. Cogswell, Carol J. Cogswell, Chrysanthe Preza, Chrysanthe Preza, } "Quantitative phase microscopy through differential interference imaging," Journal of Biomedical Optics 13(2), 024020 (1 March 2008). https://doi.org/10.1117/1.2907328 . Submission:
JOURNAL ARTICLE
10 PAGES


SHARE
Back to Top