Measurements of light scattering in an integrated microfluidic waveguide cytometer

Xuan-Tao Su; Kirat Singh; Clarence E. Capjack; Jiri Petracek; Christopher J. Backhouse; Wojciech Rozmus

doi:10.1117/1.2909670

1 March 2008 Measurements of light scattering in an integrated microfluidic waveguide cytometer

Xuan-Tao Su; Kirat Singh; Clarence E. Capjack; Jiri Petracek; Christopher J. Backhouse; Wojciech Rozmus

Author Affiliations +

J. of Biomedical Optics, 13(2), 024024 (2008). https://doi.org/10.1117/1.2909670

Abstract

An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.

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Xuan-Tao Su, Xuan-Tao Su, Kirat Singh, Kirat Singh, Clarence E. Capjack, Clarence E. Capjack, Jiri Petracek, Jiri Petracek, Christopher J. Backhouse, Christopher J. Backhouse, Wojciech Rozmus, Wojciech Rozmus, } "Measurements of light scattering in an integrated microfluidic waveguide cytometer," Journal of Biomedical Optics 13(2), 024024 (1 March 2008). https://doi.org/10.1117/1.2909670 . Submission:

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