1 July 2008 Extended cavity laser enhanced two-photon flow cytometry
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J. of Biomedical Optics, 13(4), 041319 (2008). doi:10.1117/1.2967983
Abstract
We demonstrate enhanced sensitivity in two-photon flow cytometry with an extended cavity laser excitation source. At low power, the home-built 20-MHz oscillator was able to detect a significantly larger fraction, in either phosphate buffered saline (PBS) or whole blood, of green fluorescent protein (GFP)–expressing MCA-207 cells cross-labeled with the membrane-binding lipophilic dye DiD. A geometrical model is used to explain unique features of the signals resulting from the different spatial distribution of DiD and GFP. These unique features include sub-square law scaling of unsaturated two-photon signal, a sigmoidal sensitivity curve for detection under varying powers for cell detection thresholds as low as a single photon, and uncorrelated signal strengths in two detection channels.
Eric R. Tkaczyk, Alan H. Tkaczyk, Steve M. Katnik, Jing Yong Ye, Kathryn E. Luker, Gary D. Luker, Andrzej Myc, James R. Baker, Jr., Theodore B. Norris, "Extended cavity laser enhanced two-photon flow cytometry," Journal of Biomedical Optics 13(4), 041319 (1 July 2008). http://dx.doi.org/10.1117/1.2967983
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KEYWORDS
Green fluorescent protein

Blood

Signal detection

Flow cytometry

Capillaries

In vivo imaging

Optical spheres

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