1 July 2008 Characterization of optical-aberration-induced lateral and axial image inhomogeneity in multiphoton microscopy
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Abstract
The effects of off-axis optical aberration in multiphoton microscopy and the resulting lateral and axial image inhomogeneity are investigated. The lateral inhomogeneity of the scanning field is demonstrated by second harmonic generation (SHG) imaging of fasciae and two-photon fluorescence (TPF) microscopy of thin fluorescent samples. Furthermore, refractive index mismatch-caused intensity attenuation of the TPF signal at central and peripheral regions of the scanning frame is measured using homogeneous 10-μM sulforhodamine B samples with refractive indexes of 1.33 and around 1.465. In addition to characterizing image field convexity, we also found that image resolution degrades away from the optical axis. These effects need to be accounted for in both qualitative and quantitative multphoton imaging applications.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Vladimir A. Hovhannisyan, Vladimir A. Hovhannisyan, Ping-Jung Su, Ping-Jung Su, Chen-Yuan Dong, Chen-Yuan Dong, } "Characterization of optical-aberration-induced lateral and axial image inhomogeneity in multiphoton microscopy," Journal of Biomedical Optics 13(4), 044023 (1 July 2008). https://doi.org/10.1117/1.2950314 . Submission:
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