1 July 2008 Raman microscopy for dynamic molecular imaging of living cells
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Abstract
We demonstrate dynamic imaging of molecular distribution in unstained living cells using Raman scattering. By combining slit-scanning detection and optimizing the excitation wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found that 532-nm excitation can be used to generate strong Raman scattering signals and to suppress autofluorescence that typically obscures Raman signals. With this technique, we reveal time-resolved distributions of cytochrome c and other biomolecules in living cells in the process of cytokinesis without the need for fluorescent labels or markers.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Keisaku Hamada, Katsumasa Fujita, Nicholas Isaac Smith, Minoru Kobayashi, Yasushi Inouye, Satoshi Kawata, "Raman microscopy for dynamic molecular imaging of living cells," Journal of Biomedical Optics 13(4), 044027 (1 July 2008). https://doi.org/10.1117/1.2952192 . Submission:
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