1 July 2008 Raman microscopy for dynamic molecular imaging of living cells
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Abstract
We demonstrate dynamic imaging of molecular distribution in unstained living cells using Raman scattering. By combining slit-scanning detection and optimizing the excitation wavelength, we imaged the dynamic molecular distributions of cytochrome c, protein beta sheets, and lipids in unstained HeLa cells with a temporal resolution of 3 minutes. We found that 532-nm excitation can be used to generate strong Raman scattering signals and to suppress autofluorescence that typically obscures Raman signals. With this technique, we reveal time-resolved distributions of cytochrome c and other biomolecules in living cells in the process of cytokinesis without the need for fluorescent labels or markers.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Keisaku Hamada, Keisaku Hamada, Katsumasa Fujita, Katsumasa Fujita, Nicholas Isaac Smith, Nicholas Isaac Smith, Minoru Kobayashi, Minoru Kobayashi, Yasushi Inouye, Yasushi Inouye, Satoshi Kawata, Satoshi Kawata, } "Raman microscopy for dynamic molecular imaging of living cells," Journal of Biomedical Optics 13(4), 044027 (1 July 2008). https://doi.org/10.1117/1.2952192 . Submission:
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