1 September 2008 In vitro model that approximates retinal damage threshold trends
Author Affiliations +
J. of Biomedical Optics, 13(5), 054014 (2008). doi:10.1117/1.2981831
Without effective in vitro damage models, advances in our understanding of the physics and biology of laser-tissue interaction would be hampered due to cost and ethical limitations placed on the use of nonhuman primates. We extend our characterization of laser-induced cell death in an existing in vitro retinal model to include damage thresholds at 514 and 413 nm. The new data, when combined with data previously reported for 532 and 458 nm exposures, provide a sufficiently broad range of wavelengths and exposure durations (0.1 to 100 s) to make comparisons with minimum visible lesion (in vivo) data in the literature. Based on similarities between in vivo and in vitro action spectra and temporal action profiles, the cell culture model is found to respond to laser irradiation in a fundamentally similar fashion as the retina of the rhesus animal model. We further show that this response depends on the amount of intracellular melanin pigmentation.
Michael L. Denton, Michael S. Foltz, Kurt J. Schuster, Gary D. Noojin, Larry E. Estlack, Robert J. Thomas, "In vitro model that approximates retinal damage threshold trends," Journal of Biomedical Optics 13(5), 054014 (1 September 2008). http://dx.doi.org/10.1117/1.2981831

In vitro testing

Animal model studies

Data modeling

Laser damage threshold

Eye models

In vivo imaging

Beam shaping


Photonic limiters with enhanced dynamic range
Proceedings of SPIE (January 01 2018)
Galvanometer Compensation To Extend Its Frequency Range
Proceedings of SPIE (January 10 1977)
The Changing Ophthalmic Marketplace
Proceedings of SPIE (September 06 1988)

Back to Top