1 September 2008 Fibroblast autofluorescence in connective tissue disorders: a future tool for clinical and differential diagnosis?
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J. of Biomedical Optics, 13(5), 054025 (2008). doi:10.1117/1.2982533
Abstract
Marfan syndrome (MFS) is an inherited disorder of connective tissue due to mutations in FBN1 (90%) and TGFBR1 and TGFBR2 (5 to 10%) genes. Clinical and differential diagnosis is difficult because of the inter- and intrafamiliar marked heterogeneity and the variable onset age of clinical manifestations. Among the disorders, in differential diagnosis, thoracic aortic aneurysm (TAA) and Ullrich scleroatonic muscular dystrophy (UCMD) are reported. We evaluate the possibility of utilizing autofluorescence (AF) analysis as a diagnostic tool in the clinical and/or differential diagnosis of MFS and related disorders and in the investigation of the molecular mechanisms involved. Both multispectral imaging autofluorescence microscopy (MIAM) and autofluorescence microspectroscopy (AMS) have been used to characterize AF emission of fibroblasts from patients affected by inherited connective tissue disorders. Our preliminary results show significant differences in AF emission between normal and pathological fibroblasts, suggesting possible improvement in diagnostics of connective tissue disorders by AF analysis.
Monica Monici, Venere Basile, Giovanni Romano, Lucia Evangelisti, Laura Lucarini, Monica Attanasio, Enrico Bertini, Franco Fusi, Gian Franco Gensini, Guglielmina Pepe, "Fibroblast autofluorescence in connective tissue disorders: a future tool for clinical and differential diagnosis?," Journal of Biomedical Optics 13(5), 054025 (1 September 2008). http://dx.doi.org/10.1117/1.2982533
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KEYWORDS
Atrial fibrillation

Connective tissue

Luminescence

Diagnostics

Multispectral imaging

Mode conditioning cables

Optical filters

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