Light scattering by neutrophils: model, simulation, and experiment

Darya Yu. Orlova; Maxim A. Yurkin; Alfons G. Hoekstra; Valerii Pavlovich Maltsev

doi:10.1117/1.2992140

1 September 2008 Light scattering by neutrophils: model, simulation, and experiment

Darya Yu. Orlova; Maxim A. Yurkin; Alfons G. Hoekstra; Valerii Pavlovich Maltsev

Author Affiliations +

J. of Biomedical Optics, 13(5), 054057 (2008). https://doi.org/10.1117/1.2992140

Abstract

We studied the elastic light-scattering properties of human blood neutrophils, both experimentally and theoretically. The experimental study was performed with a scanning flow cytometer measuring the light-scattering patterns (LSPs) of individual cells over an angular range of 5–60 deg. We determined the absolute differential light-scattering cross sections of neutrophils. We also proposed an optical model for a neutrophil as a sphere filled by small spheres and prolate spheroids that correspond to granules and segmented nucleus, respectively. This model was used in simulations of LSPs using the discrete dipole approximation and different compositions of internal organelles. A comparison of experimentally measured and simulated LSPs gives a good qualitative agreement in LSP shape and quantitative agreement in overall magnitude of the differential light-scattering cross section.

Citation Download Citation

Darya Yu. Orlova, Darya Yu. Orlova, Maxim A. Yurkin, Maxim A. Yurkin, Alfons G. Hoekstra, Alfons G. Hoekstra, Valerii Pavlovich Maltsev, Valerii Pavlovich Maltsev, } "Light scattering by neutrophils: model, simulation, and experiment," Journal of Biomedical Optics 13(5), 054057 (1 September 2008). https://doi.org/10.1117/1.2992140 . Submission:

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $20.00

Non-members: $25.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years