1 September 2008 Design and optimization of a high-speed, high-sensitivity, spinning disk confocal microscopy system
Author Affiliations +
Abstract
We describe the principles, design, and systems integration of a flexible, high-speed, high-sensitivity, high-resolution confocal spinning disk microscopy (SDCM) system. We present several artifacts unique to high-speed SDCM along with techniques to minimize them. We show example experimental results from a specific implementation capable of generating 3-D image stacks containing 30 2-D slices at 30 stacks per second. This implementation also includes optics for differential interference contrast (DIC), phase, and bright-field imaging, as well as an optical trap with sensitive force and position measurement.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Ryan McAllister, Daniel R. Sisan, Jeffrey S. Urbach, "Design and optimization of a high-speed, high-sensitivity, spinning disk confocal microscopy system," Journal of Biomedical Optics 13(5), 054058 (1 September 2008). https://doi.org/10.1117/1.2992141 . Submission:
JOURNAL ARTICLE
7 PAGES


SHARE
Back to Top