1 September 2008 High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas
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Abstract
A predicament when assessing the mechanisms underlying the pathogenesis of type-1 diabetes (T1D) has been to maintain simultaneous global and regional information on the loss of insulin-cell mass and the progression of insulitis. We present a procedure for high-resolution 3-D analyses of regions of interest (ROIs), defined on the basis of global assessments of the 3-D distribution, size, and shape of molecularly labeled structures within the full volume of the intact mouse pancreas. We apply a refined protocol for optical projection tomography (OPT)-aided whole pancreas imaging in combination with confocal laser scanning microscopy of site-directed pancreatic microbiopsies. As such, the methodology provides a useful tool for detailed cellular and molecular assessments of the autoimmune insulitis in T1D. It is anticipated that the same approach could be applied to other areas of research where 3-D molecular distributions of both global and regional character is required.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Tomas Alanentalo, Tomas Alanentalo, Christina E. Loren, Christina E. Loren, Asa Larefalk, Asa Larefalk, James Sharpe, James Sharpe, Dan Holmberg, Dan Holmberg, Ulf Ahlgren, Ulf Ahlgren, } "High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas," Journal of Biomedical Optics 13(5), 054070 (1 September 2008). https://doi.org/10.1117/1.3000430 . Submission:
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