1 November 2008 Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats
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Abstract
Photodynamic therapy (PDT) is a promising cancer treatment that involves optical excitation of photosensitizers that promote oxygen molecules to the metastable O2(a1Δ) state (singlet oxygen). This species is believed to be responsible for the destruction of cancerous cells during PDT. We describe a fiber optic-coupled, pulsed diode laser-based diagnostic for singlet oxygen. We use both temporal and spectral filtering to enhance the detection of the weak O2(a→X) emission near 1.27 µm. We present data that demonstrate real-time singlet oxygen production in tumor-laden rats with chlorin e6 and 5-aminolevulinic acid-induced protoporphyrin photosensitizers. We also observe a positive correlation between post-PDT treatment regression of the tumors and the relative amount of singlet oxygen measured. These results are promising for the development of the sensor as a real-time dosimeter for PDT.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Seonkyung Lee, Danthu H. Vu, Michael F. Hinds, Steven J. Davis, Alvin Liang, Tayyaba Hasan, "Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats," Journal of Biomedical Optics 13(6), 064035 (1 November 2008). https://doi.org/10.1117/1.3042265 . Submission:
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