1 November 2008 Monitoring angiogenesis noninvasively with near-infrared spectroscopy
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Near-infrared (NIR) spectroscopy is used to quantify cerebral blood volume (CBV) as a marker of angiogenesis (formation of new blood vessels). Rats are exposed to chronic hypoxia for 3 weeks at half atmospheric pressure to stimulate angiogenesis, and second-differential NIR spectroscopy is used to quantify total cerebral hemoglobin before and after angiogenesis. The cerebral hemoglobin (from broadband NIR spectroscopy), and the large vessel hemoglobin and hematocrit (from blood samples), are used to derive values for the calculation of CBV. The total hemoglobin in brain is 46.6±1.9 μmol/l (mean±SD, n=5) preacclimation and increases by 72% postacclimation. CBV is initially 3.26±0.41% v/v and increases by 31% with acclimation. Each individual animal shows a measureable increase in CBV. This study indicates that NIR broadband spectroscopy can be used for repeated measurements of CBV and can be applied as a noninvasive method to study angiogenesis.
© (2008) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jeffrey F. Dunn, Jeffrey F. Dunn, Qiong Zhang, Qiong Zhang, Ying Yu, Ying Yu, Sathyanarayanan Srinivasan, Sathyanarayanan Srinivasan, Michael R. Smith, Michael R. Smith, R. Anthony Shaw, R. Anthony Shaw, } "Monitoring angiogenesis noninvasively with near-infrared spectroscopy," Journal of Biomedical Optics 13(6), 064043 (1 November 2008). https://doi.org/10.1117/1.3000431 . Submission:

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