1 January 2009 Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media
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J. of Biomedical Optics, 14(1), 010508 (2009). doi:10.1117/1.3081544
Abstract
We present a comparative study of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) imaging in turbid media at 800- and 1300-nm excitation. The depth-dependent decay of TPEF and SHG signals in turbid tissue phantoms is used to estimate the impact of light scattering on excitation intensity at each wavelength. A 50 to 80% increase in scattering length is observed using 1300-nm excitation, while peak TPEF emission intensity is obtained 10 to 20 μm beneath the surface for both sources. The increased penetration depth at 1300 nm is confirmed by TPEF and SHG microscopy of tissue phantoms composed of gelatin/microspheres and 3-D organotypic collagen-fibroblast cultures, respectively. Our results establish the feasibility of 1.3-μm excitation in nonlinear optical microscopy.
Mihaela Balu, Tommaso Baldacchini, John L. Carter, Tatiana B. Krasieva, Ruben Zadoyan, Bruce Jason Tromberg, "Effect of excitation wavelength on penetration depth in nonlinear optical microscopy of turbid media," Journal of Biomedical Optics 14(1), 010508 (1 January 2009). http://dx.doi.org/10.1117/1.3081544
Submission: Received ; Accepted
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KEYWORDS
Scattering

Second-harmonic generation

Microscopy

Photons

Laser scattering

Tissues

Collagen

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