1 May 2009 Fabrication of indocyanine green encapsulated biodegradable microbubbles for structural and functional imaging of cancer
Author Affiliations +
J. of Biomedical Optics, 14(3), 034020 (2009). doi:10.1117/1.3147424
Abstract
We developed a novel dual-modal contrast agent for the structural and functional imaging of cancer. The contrast agent was fabricated by encapsulating indocyanine green (ICG) in poly(lactic-co-glycolic acid) (PLGA) microbubbles using a modified double-emulsion method. More stabilized absorption and fluorescence emission characteristics were observed for aqueous and plasma suspensions of ICG-encapsulated microbubbles. The technical feasibility of concurrent structural and functional imaging was demonstrated through a series of benchtop tests in which the aqueous suspension of ICG-encapsulated microbubbles was injected into a transparent tube embedded in an Intralipid phantom at different flow rates and concentrations. Concurrent fluorescence imaging and B-mode ultrasound imaging successfully captured the changes of microbubble flow rate and concentration with high linearity and accuracy. One potential application of the proposed ICG-encapsulated PLGA microbubbles is for the identification and characterization of peritumoral neovasculature for enhanced coregistration between tumor structural and functional boundaries in ultrasound-guided near-infrared diffuse optical tomography.
Ronald X. Xu, Jiwei Huang, Jeff S. Xu, Duxin Sun, George H. Hinkle, Edward W. M. Martin, Stephen P. Povoski, "Fabrication of indocyanine green encapsulated biodegradable microbubbles for structural and functional imaging of cancer," Journal of Biomedical Optics 14(3), 034020 (1 May 2009). http://dx.doi.org/10.1117/1.3147424
Submission: Received ; Accepted
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KEYWORDS
Tumors

Luminescence

Plasma

Absorption

Near infrared

Ultrasonography

Indocyanine green

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