1 May 2009 High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells
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J. of Biomedical Optics, 14(3), 034030 (2009). doi:10.1117/1.3155518
Abstract
Diffusion-time distribution analysis (DDA) has been used to explore the plasma membrane fluidity of multidrug-resistant cancer cells (LR73 carcinoma cells) and also to characterize the influence of various membrane agents present in the extracellular medium. DDA is a recent single-molecule technique, based on fluorescence correlation spectroscopy (FCS), well suited to retrieve local organization of cell membrane. The method was conducted on a large number of living cells, which enabled us to get a detailed overview of plasma membrane microviscosity, and plasma membrane micro-organization, between the cells of the same line. Thus, we clearly reveal the higher heterogeneity of plasma membrane in multidrug-resistant cancer cells in comparison with the nonresistant ones (denoted sensitive cells). We also display distinct modifications related to a membrane fluidity modulator, benzyl alcohol, and two revertants of multidrug resistance, verapamil and cyclosporin-A. A relation between the distribution of the diffusion-time values and the modification of membrane lateral heterogeneities is proposed.
Céline C. B. Boutin, Yann Roche, Christine Millot, Regis Deturche, Pascal Royer, Michel Manfait, Jérôme Plain, Pierre Jeannesson, Jean-Marc Millot, Rodolphe Jaffiol, "High heterogeneity of plasma membrane microfluidity in multidrug-resistant cancer cells," Journal of Biomedical Optics 14(3), 034030 (1 May 2009). http://dx.doi.org/10.1117/1.3155518
Submission: Received ; Accepted
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KEYWORDS
Plasma

Diffusion

Fluorescence correlation spectroscopy

Cancer

Microfluidics

Molecules

Luminescence

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