1 July 2009 Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes
Author Affiliations +
J. of Biomedical Optics, 14(4), 040506 (2009). doi:10.1117/1.3207151
Abstract
We demonstrate that stimulated parametric emission (SPE) microscopy enables label-free, 3-D visualization of internal hemoglobin distribution of live mouse and chicken erythrocytes with high sensitivity. Change in hemoglobin distribution in chicken erythrocytes before and after ethanol fixation is clearly visualized.
Hieu M. Dang, Gen Omura, Toshiyuki Omano, Masatomo Yamagiwa, Shin'ichiro Kajiyama, Yasuyuki Ozeki, Kazuyoshi Itoh, Kiichi Fukui, "Label-free imaging by stimulated parametric emission microscopy reveals a difference in hemoglobin distribution between live and fixed erythrocytes," Journal of Biomedical Optics 14(4), 040506 (1 July 2009). http://dx.doi.org/10.1117/1.3207151
Submission: Received ; Accepted
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KEYWORDS
Microscopy

Visualization

Spatial light modulators

Signal processing

Optical filters

Dispersion

Mirrors

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