1 November 2009 In vivo harmonic generation biopsy of human skin
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J. of Biomedical Optics, 14(6), 060505 (2009). doi:10.1117/1.3269676
Abstract
The ability to in vivo image deep tissues noninvasively with a high resolution is strongly required for optical virtual biopsy. Higher harmonic generation microscopy, combined with second- and third-harmonic generation microscopies, is applied to 17 Asian volunteers' forearm skin. After continuous observation for 30 min, no visible damage was found. Our study proves that harmonic generation biopsy (HGB) is able to satisfy the safety requirement and to provide high penetrability (~300 μm) and submicron resolution all at the same time and is a promising tool for future virtual biopsy of skin diseases. In contrast to a previous study on fixed human skin specimens, a much improved penetrability and much reduced resolution-degradation versus depth are found in this in vivo examination.
Szu-Yu Chen, Hai-Yin Wu, Chi-Kuang Sun, "In vivo harmonic generation biopsy of human skin," Journal of Biomedical Optics 14(6), 060505 (1 November 2009). http://dx.doi.org/10.1117/1.3269676
Submission: Received ; Accepted
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KEYWORDS
Skin

In vivo imaging

Biopsy

Microscopy

Second-harmonic generation

Harmonic generation

Collagen

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