1 January 2009 Two-photon imaging using adaptive phase compensated ultrashort laser pulses
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Abstract
An adaptive pulse shaper controlled by multiphoton intrapulse interference phase scanning (MIIPS) was used, together with a prism-pair, to measure and cancel high-order phase distortions introduced by a high-numerical-aperture objective and other dispersive elements of a two-photon laser-scanning microscope. The delivery of broad-bandwidth (~100 nm), sub-12-fs pulses was confirmed by interferometric autocorrelation measurements at the focal plane. A comparison of two-photon imaging with transform-limited and second-order-dispersion compensated laser pulses of the same energy showed a 6-to-11-fold improvement in the two-photon excitation fluorescence signal when applied to cells and tissue, and up to a 19-fold improvement in the second harmonic generation signal from a rat tendon specimen.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Peng Xi, Peng Xi, Yair Andegeko, Yair Andegeko, Dmitry Pestov, Dmitry Pestov, Vadim V. Lozovoy, Vadim V. Lozovoy, Marcos M. Dantus, Marcos M. Dantus, } "Two-photon imaging using adaptive phase compensated ultrashort laser pulses," Journal of Biomedical Optics 14(1), 014002 (1 January 2009). https://doi.org/10.1117/1.3059629 . Submission:
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