1 January 2009 Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques
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Abstract
We report the detection of interactions between a photosensitizer, hypericin (HY), and its solvent system prepared with a formulation additive, polyvinylpyrrolidone (PVP), a commonly used pharmaceutical excipient. Fluorescence correlation spectroscopy (FCS) and fluorescence lifetime imaging microscopy (FLIM) were used to study aggregation and binding of HY in the presence of PVP. Digitized fluorescence endoscopic imaging (DFEI) was used to study the effect of the pharmaceutical formulation in the in vivo tumor implanted chick chorioallantoic membrane (CAM) model. The results presented reveal the coordination of HY-PVP binding, HY disaggregation in the presence of PVP, and strengthened HY tumor uptake selectivity. PVP is thus suggested as a potential adjuvant to previously investigated N-methyl pyrrolidone (NMP) in the HY delivery system as well as a replacement for the conventionally used albumin in the HY bladder instillation fluids preparation for clinical use.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Jun Liu, Jun Liu, Constance Lay Lay Saw, Constance Lay Lay Saw, Malini Olivo, Malini Olivo, Thankiah Sudhaharan, Thankiah Sudhaharan, Sohail Ahmed, Sohail Ahmed, Paul Wan Sia Heng, Paul Wan Sia Heng, Thorsten Wohland, Thorsten Wohland, } "Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques," Journal of Biomedical Optics 14(1), 014003 (1 January 2009). https://doi.org/10.1117/1.3067726 . Submission:
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