1 January 2009 Automated three-dimensional tracking of living cells by digital holographic microscopy
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Abstract
Digital holographic microscopy (DHM) enables a quantitative multifocus phase contrast imaging that has been found suitable for technical inspection and quantitative live cell imaging. The combination of DHM with fast and robust autofocus algorithms enables subsequent automated focus realignment by numerical propagation of the digital holographically reconstructed object wave. In combination with a calibrated optical imaging system, the obtained propagation data quantify axial displacements of the investigated sample. The evaluation of quantitative DHM phase contrast images also enables an effective determination of lateral cell displacements. Thus, 3-D displacement data are provided. Results from investigations on sedimenting red blood cells and HT-1080 fibrosarcoma cells in a collagen tissue model demonstrate that DHM enables marker-free automated quantitative dynamic 3-D cell tracking without mechanical focus adjustment.
© (2009) Society of Photo-Optical Instrumentation Engineers (SPIE)
Patrik Langehanenberg, Patrik Langehanenberg, Lyubomira Ivanova, Lyubomira Ivanova, Ingolf Bernhardt, Ingolf Bernhardt, Steffi Ketelhut, Steffi Ketelhut, Angelika Vollmer, Angelika Vollmer, Dieter Dirksen, Dieter Dirksen, Georgi K. Georgiev, Georgi K. Georgiev, Gert von Bally, Gert von Bally, Björn Kemper, Björn Kemper, } "Automated three-dimensional tracking of living cells by digital holographic microscopy," Journal of Biomedical Optics 14(1), 014018 (1 January 2009). https://doi.org/10.1117/1.3080133 . Submission:
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